The Jefferson Street Historic District is a linear neighborhood that extends along East Jefferson Street from Clinton to Van Buren streets. Properties facing the intersecting streets of Dubuque, Linn, Gilbert, and Van Buren are also included within the district. The district includes a mix of institutional buildings (religious and academic) and residential buildings that reflect its historical development along the edge of the downtown and the University campus. University-related resources include buildings originally used as a biological sciences classroom building, a medical school anatomy lecture hall, an isolation hospital, and sorority houses. Buildings used for religious purposes include four churches, a student center, a former convent, and a rectory. The balance of the district includes two large apartment buildings, a collection of medium- and large-sized single-family dwellings that date from the 1850s through the 1930s, and a variety of secondary structures erected during the early 20th century. The district contains a total of 38 primary resources with all but one considered contributing. Buildings in the Jefferson Street Historic District exhibit a range of late 19th and early 20th century architectural styles including excellent examples of eleven distinct styles and several vernacular residential forms.